Induction of muscle-relaxing factor by staphylococcal alpha-toxin
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چکیده
منابع مشابه
Platelet damaging factor, a fifth activity of staphylococcal alpha-toxin.
Crude and purified staphylococcal alpha-toxin were used to demonstrate that the platelet-damaging effect of crude alpha-toxin represents a fifth activity of the alpha-toxin molecule. The homogeneity of the purified toxin employed was demonstrated by ultracentrifugation, Ouchterlony, and immunoelectrophoretic methods. Continuous-flow electrophoretic migration studies demonstrated under a variety...
متن کاملPurification of staphylococcal alpha-toxin by adsorption chromatography on glass.
Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chromatography, is composed of a single electrophoretic form, containing less than 2...
متن کاملUltracentrifugal analysis of staphylococcal alpha toxin.
Ultracentrifugal examination of staphylococcal alpha toxin at different stages of purification showed the presence of a major component having a sedimentation coefficient of 2.8S, present to the extent of more than 90% of the sample, and identifiable with active toxin. Several minor components having S(20,w) values of 11.5S, 8.5S, and 2.0S were detected. The 11.5S component presumably is identi...
متن کاملSoluble Relaxing Factor from Muscle*
Gergely, Briggs, and Kaldor (24) have recently shown that the so called Marsh-Bendall relaxing factor consists of a granular component and a heat labile dialyzable cofactor. The requirement for both components could be demonstrated for the inhibition of Mg++-activated myofibrillar adenosine triphosphatase, for the inhibition of the adenosine triphosphate-induced syneresis of myofibrils and for ...
متن کاملThe H35A mutated alpha-toxin interferes with cytotoxicity of staphylococcal alpha-toxin.
Staphylococcal alpha-toxin is an important virulence factor for Staphylococcus aureus to cause severe infections. In this study, we explored whether the toxoid of alpha-toxin may be utilized to block the toxicity of wild-type alpha-toxin. We created a series of H35A mutated alpha-toxin expression strains and revealed that the H35A mutation eliminates the activity of alpha-toxin using a human lu...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1994
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.62.2.421-425.1994